Sophisticated Image Analysis Algorithms
Orbit has many build-in image analysis algorithms. Tissue quantification using machine learning techniques, object / cell segmentation, and object classification are the basic ones. Region of interest (ROI) can be defined by manual annotations or via a trainable exclusion map. Everything can be combined.
WORKS FOR BIG IMAGES, e.g. WHOLE SLIDE SCANS
All algorithms are build to work on really big images, up to gigapixel images, especially whole slide scans.
This is possible due to a tile-based processing pipeline and combination with the use of different resolutions of the image.
Connectivity: OMERO & Spark connectors
You can use your existing image server, e.g. it has been designed to work great with Omero.
Orbit can also run in stand-alone mode and open whole slide images like SVS, NDPI, SCN, ….
A Spark infrastructure can be used as scaleout infrastructure to distribute computation intensive tasks.
Scripting & Extentions & Connectors
Orbit provides a versatile API for developers to create scripts or extentions. For instance, you can easily iterate over all tiles within a defined ROI and apply any algorithm or ImageJ plugin. You write s.th. which works for small in-memory images, Orbit takes care that is works for really big images.
Individual image server and scaleout connectors can also be created by implementing clear interfaces.
What it is
And it will stay for free.
No demo, it's a full version.
Orbit Image Analysis is a free open source software with the focus to quantify big images like whole slide scans.
It can connect to image servers, e.g. Omero.
Analysis can be done on your local computer or via scaleout functionality in a distrubuted computing environment like a Spark cluster.
Sophisticated image analysis algorithms incl. tissue quantification using machine learning, object segmentation and classification are build in. In addition a versatile API allows you to enhance Orbit and to run your own scripts.
Annotations & ROI
Segmenting cells in cell clusters is very challenging because they cannot be separated easily from the background – thus classical segmentation algorithms fail. Unfortunately in WSI (whole slide imaging) you will have to deal with these situations – especially if you don’t have a single cell layer, like e.g. in tumor tissue. A collaboration with[…]
Today we release Orbit version 2.52 (for Omero 5.2.x) and Orbit 2.53 (for Omero 5.3.x). Both versions are identical except support for different Omero major versions and support multichannel / fluorescence images with unlimited number of channels. In addition, this version provides many bugfixes and speed improvements. Multi series / scenes can be selected in[…]
Orbit 2.4.3 makes use of the new Bio-Formats 5.3.0 library which enables CZI files with JPEG-XR compression support. In addition Orbit supports multi image series, e.g. you can open all image series of your VSI images.
Today the new Orbit version 2.41 is released. The main achievement of this major version is the ability to work with whole slide images images in standalone mode, even without using an image server. In addition, many speed improvements (faster rendering) and bug-fixes are included. Orbit is designed to work with an image server, e.g.[…]
Orbit Image Analysis and all its dependencies are now available via Maven Central. If you want to use the Orbit API, just add <dependency> <groupId>com.actelion.research</groupId> <artifactId>orbit-image-analysis</artifactId> <version>2.30</version> </dependency> in your POM or compile ‘com.actelion.research:orbit-image-analysis:2.30’ for Gradle. See the Orbit API page for details.
Orbit integrates Cell Profiler (CP). This means CP can be used for whole slide image analysis, in combination with Orbit exclusion maps and manual defined ROIs. You can use it to segment cells and combine it with tissue quantification to e.g. count stained cells and normalize it to the ROI area or stained cell area.[…]
currently I’m preparing Orbit for the open-source release… Stay Tuned!